flow cytometry  (Danaher Inc)

Journal: Poultry Science

Article Title: miR-1737 targets TAK1 to mediate the BMP-Smad signaling pathway to regulate the molecular mechanism of chicken tibial chondrodysplasia

doi: 10.1016/j.psj.2025.106102

Figure Lengend Snippet: miR-1737 promotes the proliferation of TD chondrocytes. (A, B) Expression of miR-1737 in cartilage tissues and chondrocytes of normal group and TD Groups ( n = 6). (C, D) Detection of miR-1737 overexpression efficiency and interference efficiency. (E, F) The impact of miR-1737 overexpression and interference on the expression of proliferation-related genes ( n = 6). (G, H) CCK-8 analysis after treatment with miR-1737 mimics or inhibitor during TD chondrocytes proliferation ( n = 3). (I, J) The proliferation of chondrocytes after miR-1737 transfection was detected by EdU staining. The cells in S-phase were stained with EdU (red fluorescence) and nuclei with Hoechst (blue) and counted with Image J. The scale bar represents 200 μm. (K, L) The cell cycle of miR-1737 transfected chondrocyte was assayed by flow cytometry. We determined the proportion of cells in the G0/G1, S, and G2/M phases, respectively ( n = 3). Data were indicated as mean±SEM. * P < 0.05, ** P < 0.01, ns P ≥ 0.05.

Article Snippet: Finally, cell cycle distribution was analyzed using a Cytoflex flow cytometry (Beckman, USA), with each treatment group containing three biological replicates.

Techniques: Expressing, Over Expression, CCK-8 Assay, Transfection, Staining, Fluorescence, Flow Cytometry

Journal: Poultry Science

Article Title: miR-1737 targets TAK1 to mediate the BMP-Smad signaling pathway to regulate the molecular mechanism of chicken tibial chondrodysplasia

doi: 10.1016/j.psj.2025.106102

Figure Lengend Snippet: TAK1 inhibits the proliferation and differentiation of TD chondrocytes. (A, B) Expression of TAK1 in cartilage tissues and chondrocytes of normal group and TD Groups ( n = 6). (C, D) Detection of TAK1 overexpression efficiency and interference efficiency. (E, F) CCK-8 analysis after treatment with TAK1 mimics or inhibitor during TD chondrocytes proliferation ( n = 6). (G, H) EdU staining was conducted on chondrocytes after 24 h transfection with TAK1 overexpression and interference vectors. The scale bar represents 200 μm ( n = 6). (I, J) The impact of TAK1 overexpression and interference on the expression of proliferation-related genes ( n = 6). (K, L) The cell cycle of TAK1 transfected chondrocyte was assayed by flow cytometry. We determined the proportion of cells in the G0/G1, S, and G2/M phases, respectively ( n = 3). (M, N) The impact of TAK1 overexpression and interference on the expression of early- and late- stage chondrocyte differentiation factors ( n = 6). (O, P) The relative protein expression levels of differentiation factors Col I, Col II and Col X in chondrocytes following TAK1 overexpression or interference ( n = 3). Data were indicated as mean±SEM. * P < 0.05, ** P < 0.01, ns P ≥ 0.05.

Article Snippet: Finally, cell cycle distribution was analyzed using a Cytoflex flow cytometry (Beckman, USA), with each treatment group containing three biological replicates.

Techniques: Expressing, Over Expression, CCK-8 Assay, Staining, Transfection, Flow Cytometry

Journal: bioRxiv

Article Title: Elemental Composition and Degradation Rate Impact the Biocompatibility of Copper Chalcogenide Nanocrystals

doi: 10.64898/2025.12.17.695045

Figure Lengend Snippet: ( A ) Flow cytometry staining of HepG 2 cells with apoptosis and necrosis indicators Apopxin Red and Nuclear Green DSC1, respectively, following 6 hr incubation with 150 µg/mL copper chalcogenide NCs (cation concentration). ( B ). Summary and statistical analysis of the necrosis assay (n = 3). * p < 0.05, **** p < 0.0001.

Article Snippet: The cells were measured using a multi-channel CytoFLEX S flow cytometry analyzer (Beckman Coulter, CA, USA).

Techniques: Flow Cytometry, Staining, Incubation, Concentration Assay